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Inventi Impact: Animal Models & Cell Assays

Articles

  • Inventi:pac/14524/14
    Using a Medium-throughput Comet Assay to Evaluate the Global DNA Methylation Status of Single\nCells
    01-Jan-1970 Research 2014 : October - December
    Angelique Lewies, Etresia Van Dyk, Johannes F Wentzel, Pieter J Pretorius

    The comet assay is a simple and cost effective technique, commonly used to analyze\nand quantify DNA damage in individual cells. The versatility of the comet assay allows\nintroduction of various modifications to the basic technique. The difference in the\nmethylation sensitivity of the isoschizomeric restriction enzymes HpaII and MspI are used\nto demonstrate the ability of the comet assay to measure the global DNA methylation level\nof individual cells when using cell cultures. In the experiments described here, a mediumthroughput\ncomet assay and methylation sensitive comet assay are combined to produce\na methylation sensitive medium-throughput comet assay to measure changes in the global\nDNA methylation pattern in individual cells under various growth conditions.

    How to Cite this Article
    CC Compliant Citation: Lewies A, Van Dyk E, Wentzel J F and Pretorius P J (2014) Using a medium-throughput comet assay to evaluate the\nglobal DNA methylation status of single cells. Front. Genet. 5:215. doi: 10.3389/fgene.2014.00215. Copyright © 2014 Lewies, Van Dyk, Wentzel and Pretorius. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) \n(CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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